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Rates of respiration were determined before and after addition of 1.5 mM ADP (State 2 and State 3 respectively) or at an intermediate rate (State 3.5 – isolated mitochondria only) established by addition of ATP and creatine in the presence of 160 μg of creatine kinase per mg mitochondria to mimic ATP turnover in vivo.
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Denudation accompanies uplift and may reduce rock mass in the orogenic system in the long run, but whether the total mass is growing, shrinking, or static depends on the budget established by additions from subduction versus losses from erosion.
We have shown previously that a reducing pathway can be re-established by addition of GSH to SP cells resulting in re-equilibration of the PrxIV redox status to reflect that seen for intact cells (Tavender and Bulleid, 2010b).
The integrity of the outer mitochondrial membrane was established by the addition of cytochrome c (19 μmol/l); no stimulation of respiration was observed.
These FRET experiments can show dynamics in real-time; for example, a baseline with normal acetyltransferase/deacetylase activity levels is established, followed by addition of drug, and the time-course of drug response is thereby followed in each cell.
14 As such, given the time horizon of this study and available data, continued work is warranted to study the impact of more recent APAP dose limitations established by the FDA in addition to studies focusing directly on consumer perceptions, attitudes, beliefs, knowledge and health literacy.
In all cases, uniformity of analytical response was established by the method of additions.
end{aligned} (2.1) The dual Knesser-Süss inequality for the radial Blaschke addition was established by Lutwak [1].
Given the expanded fanbase established by the 2009 film, and the addition of Sherlock star Benedict Cumberbatch as the Star Fleet's formidable new foe, it's no big surprise to see numbers rise.
However, non-Isma'ili ideas can also be found in the work, for example the invitation to celibacy proper of Sufism and the mention of feasts linked to Sabean rituals in addition to those established by Muslim law.
In addition, zygosity was established by using DNA in a sample of 311 twin pairs who could not be classified by their questionnaire responses.
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