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In nonmetastatic breast cancer patients, a cut-off at one CTC is established based on previous trials [ 29- 31], therefore, we analyzed the prognostic value of CTCs by using this cut-off in stage III IBC patients as well.
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This assay was established based on previous studies using a HUVEC migration assay [ 35– 35].
The cut of values are established based on previous literature [ 29].
The mechanism of pH-triggered release from nanocarriers based on polyelectrolytes was well established based on previous reports.
The optimal concentrations (30 nM), and conditions for siRNA transfection was established based on previous dose response studies.
These criteria have been established based on previous testing of a control cohort of healthy volunteers (n = 381).
The dose of sodium cromolyn was established based on a previous study [ 6].
Filter limits have been chosen based on previous trials where there were no other wireless nodes interfering.
Based on previous trials, like the PRORATA-study [ 17], we assumed a 28% mortality rate in each group.
Previous MI, ischemic stroke or previous revascularization was established based on medical records.
Prior diagnosis of PAD was established based on ultrasonographic or angiographic results or, previous vascular surgical or endovascular revascularizations.
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