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BT-474 (estrogen receptor (ER -positive), ZR-75-1 (ER -positive, and MDA-MB-231 (ER-negative), cells were established as subcutaneous xenografts by the injection of 107 cells suspended in 150 μl of Matrigel (Becton Dickinson Labware, Bedford, MA, USA) in the lateral back region.
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CMeC-1 and LMeC cell lines were selected, following the preliminary studies on the four canine melanoma lines, due to their ability to establish as subcutaneous tumours in CD1 Nu/Nu immunocompromised mice.
PAC120 was established as xenograft by serial subcutaneous grafting of prostate cancer tissue into 8-10-week-old Swiss nu/nu male mice (bred and maintained at the Institut Curie SPF animal facility), as published (de Pinieux et al, 2001).
In contrast, mhATF and the human endostatin even at higher molecular plasma concentration than the mhATF-BPTI did not achieve any significant inhibition of tumour growth as in established subcutaneous tumours as well as in micrometastasis in the lungs.
CALS and CALR subcutaneous tumours were established as described previously (Box et al, 2013a).
Subcutaneous tumour growth was established as previously described [3].
In the in vivo regulation assay, mice were obtained from Taconic (Ejby, Denmark), subcutaneous C33A cell tumors were established as above, and treated with a single intravenous injection of 1×1011 vp on day 1.
2 Despite the proven efficacy, enfuvirtide was not established as a routinely used drug because of its application mode as subcutaneous injections twice daily and the resultant side effects predominantly present as cutaneous irritations and painful indurations.
In contrast to multiple sclerosis, the etiology of EAE is well established as is it induced by the intravenous transfer of activated neuro-antigen-specific T cell blasts or by subcutaneous immunization with myelin antigens in susceptible animals.
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