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The specific surface area for fractions >75 μm and <75 μm were 0.42 and 1.65 m/g and were established as low and high levels respectively.
The safety of LCn-3 supplementation for doses of up to 4g of EPA & DHA/day has been established as low, with the most common concerns arising in regards to gastrointestinal upset and allergic reactions [ 58].
Pure distilled water as culture medium was established as low level; the use of 9 K medium [ 51], which contained (per liter of distilled water) 3.0 g of (NH4 2SO4, 0.5 g of MgSO4·7H2O, 0.1 g of KCl, 0.5 g of K2PO4, 0.01 g of Ca(NO3 2 was settled as high level.
Combined SPACE-PCR with analysis of snapback primer by high resolution melting (SPACE-HRM), the high sensitive system that enables a closed-tube detection of mutations after isolating mutants has been established, as low as 1/10 1/1000 mutant samples can be diagnosed.
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All cell lines were purchased from the American Type Culture Collection (ATCC), established as a low passage cell bank and then routinely passaged in our laboratory for less than 3 months after resuscitation.
The 'optimal' CDP was established as the lowest CDP that achieved the most favorable combination between the highest PaO2 and/or lowest PaCO2, while maintaining the FiO2 constant at 1.0.
For the azoles such as fluconazole, itraconazole and ketoconazole, the MIC was established as the lowest concentration that inhibited 80% of the control growth.
The limit of quantitation (LOQ) was established as the lowest concentration of an analyte in a sample that can be determined with acceptable precision and accuracy under the stated conditions of test [37].
The MIC was established as the lowest sample concentration that prevented visible growth [ 22].
Averaged responses to 256 stimuli, presented at 42.2/second, were analysed and thresholds established as the lowest sound intensity giving a visually detectable ABR response.
Minimum inhibitory concentration (MIC) was established as the lowest concentration of compound able to inhibit the visible growth of microorganism after overnight incubation.
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