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Hela cells established as above were plated at 1×104 cells/ well in 96-well plates and cultured with or without 0.1 µg/ml tetracycline.
In the in vivo regulation assay, mice were obtained from Taconic (Ejby, Denmark), subcutaneous C33A cell tumors were established as above, and treated with a single intravenous injection of 1×1011 vp on day 1.
GFP-Rab32, GFP-Pho1, GFP-CaM and GFP-VAMP1 cell lines were established as above with some modifications.
The data from one such experiment are shown in Figure 1c and 1d where cells were harvested on day 7 from cultures established as above for Figure 1a washed and stained for intracellular p24 as described in Materials and Methods.
Tumours were established as above for the Colo205 and HCT116 combination treatment models.
Meanwhile, pGL3-GG/TT containing rs4648065 site was established as above described method to investigate the co-effect on rs4648068.
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Co-cultures of OPCs and DRG were established, as described above.
Results were plotted in Excel and significance was established as described above.
Data was plotted in Excel and significance was established as described above.
Tumors were established as described above and were allowed to grow to 6 8 mm in diameter (120 270 mm3) before commencing treatment.
For the pharmacodynamic study, tumors were established as described above and were allowed to grow to a size of 0.5 0.8cm3 before treatment started.
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