In order to characterize each of the alleles and establish whether the mutation was monoallelic (BRAF V600DK601del ) or biallelic (BRAF V600D and BRAF K601del ), we applied a TA cloning-based sequencing method.
To establish whether the mutation status of spliceosomal genes can add to the predictive power of IPSS-R, we performed multivariable Cox regression analyses, examining age, sex, IPSS-R total score, and SF3B1/ U2AF1/ SRSF2 mutation status (Table 4).
To establish whether the mutations were in cis- or transconfiguration, a double digestion (BseYI+AciI) was performed, since we were not able to carry out the genomic analysis on patient's parents.
To establish whether the G119S mutation has arisen independently in each form or by genetic introgression, we analysed coding and non-coding sequences of ace-1 alleles in M and S mosquitoes from representative field populations.
"Despite the experimental data provided here, it is impossible to clearly establish whether the adaptive mutations observed were in part responsible for the extended duration of the 2013 16 epidemic," they write in their paper.
It is a major challenge to establish whether these mutations are fixed into a population by neutral genetic drift or via positive selection, and thereby evaluate the impact of the environment on this fixation.
In this study, a S288c background (aUHT strain) was used to select chromosomal rearrangements and a comparison of these results with those previously seen in FL100 allowed us to establish whether the genetic background affects the mutation rate and the type of rearrangements.
To establish whether the improved stability of the I539T mutant was due to rescued conformation, we in vitro translated wild-type and ΔF508 NBD1 with or without suppressor mutations and monitored changes in proteolytic digestion (Figure 4A).
To establish whether the interaction of each lobe observed was genuine or a consequence of the mutation in the other lobe, the Ca2+ dissociation kinetics was examined using CaMWT.
In order to establish whether the differential expression of some genes during production was linked to the mutations that had been accumulated during mutagenesis of QM6a to NG 14 and subsequently RUT C30, we compared the gene expression profiles obtained in this work with the mutations found in NG 14 and RUT C30 (Additional file 7: Table S4 [ 21, 22, 25, 37]).
To establish whether the catalytic activity of AP2C3 phosphatase is needed for induction of ectopic cell differentiation into stomata, we overexpressed a catalytically inactive version of AP2C3 carrying the mutation G163D (Figure S10), which is corresponding to the null mutation G139D of AtPP2CA [55].
