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They can be used to establish transcript catalogues [ 9- 11] and to provide experimental evidence when building gene models from genomic sequence, particularly for 5' and 3' untranslated sequences [ 12].
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In this study, we established transcript databases for leaves, roots and flowers from 3-year-old P. notoginseng.
In contrast, a novel acceptor downstream should be the minor splice site, consequently the established transcript is predominantly expressed.
We selected eight genes that have been shown to be involved in fin regeneration of zebrafish and identified the orthologous genes in N. furzeri using the recently established transcript catalogue (Petzold et al., 2013).
19 Once a consistent coding framework is established, transcripts will be coded using NVivo V.9 (QSR International Pty Ltd, Doncaster, Australia) software.
Although active replication of schistosome retrotransposons has not been established, transcripts encoding reverse transcriptase (RT) and endonuclease are detectable [ 10, 11, 22], as is RT activity in parasite extracts [ 23], suggesting that at least some of these elements are actively mobile within the genome.
Ten undisturbed animals were euthanized at lights on (7:00 am) to establish baseline transcript expression.
Products were assessed for their approximate quantity and sequence to ascertain their structure and thereby broadly establish the transcript repertoire.
To establish which transcripts are likely to be regulated directly by Htz1, we compared changes in transcript levels in the absence of Htz1 to Htz1 occupancy.
To establish orthologous transcripts of orthologous genes in a reliable way, it is therefore important to take into account the conservation not only in sequence but also in intron-exon structure and in splicing pattern.
This was to confirm the agarose gel findings indicating the presence or absence of each alternate first exon in the brain transcript and to establish the entire transcript for STS in brain tissue.
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