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The Zambia census of the population mapping system was used to establish the sampling frame which consisted of all 26 standard enumeration areas (SEAs) in Kapiri Mposhi (5225 households) and all 24 SEAs in Chelston (2786 households).
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A power calculation was performed a priori, to establish the sample size, using the WINPEPI software programme PAIRSetc [ 26, 27].
When designing the experiment, use Sobol's method to randomly select a series of samples as estimated input variables and establish the sample space.
A multistage sampling technique was used to establish the sample size; data were collected from 350 military and civilian cantonment residents using a pretested questionnaire.
The calculations to establish the sample size were done using the software G*Power 3.1.9.2 with one-way ANOVA test in three groups.
No definitive calculation exists to establish the sample size for a DCE 35 36; the survey ran until approximately 600 participants had completed it.
To establish the sample size for the study, a 10% difference in scores for the outcome measure assessed by questionnaires between the two arms of the study was considered clinically meaningful and assuming a standard deviation of 10 [ 36].
We next evaluated plate-to-plate and day-to-day variability by constructing a control plate containing DMSO (no PAD2) or Cl-amidine for the high controls, and DMSO (no inhibitors, low control) plus PAD2 to establish the sample field.
The first stage involved establishing the sampling frame.
Teams of medical doctors and nurses established the sampling frames and collected the information and samples in all the regions as described in Table 1.
Once established the sampling sites in which the three highest and lowest phenols values were found, shoots from these sampling sites were selected for protein analysis.
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