Sentence examples for establish reading frame from inspiring English sources
A single insert-positive clone (with pMAMneo- gef) was selected for transfection into MCF-7 cells after analysis by restriction-enzyme digestions and PCR to establish reading frame orientation and sequence fidelity.
In addition, we established reading frames from local BLASTX alignments [ 37] using the methods of Hahn et al. [ 36] to compensate for 454 under/overcall errors.
Briefly, each contig consensus was compared against the NCBI protein database using BLASTX to establish a reading frame in which to assess synonymous or non-synonymous status.
In addition to allowing ribosomal entry and accommodation, this recognition event also establishes the reading frame on tmRNA.
Seven Gs leads to a frameshift while six Gs maintains the established open reading frame in MPV17.
Previous biochemical and structural studies show that domain III is not needed for initial subunit recruitment and 80S ribosome formation but is essential for establishing the reading frame by docking precisely in the ribosome's decoding groove (Nishiyama, 2003; Costantino and Kieft, 2005; Jan and Sarnow, 2002).
The PKI pseudoknot of the CrPV-IRES is thought to mimic the initiator tRNA/mRNA interaction and thus to establish the correct reading frame in the viral messenger upon interaction with the ribosome (Costantino et al., 2008).
The SmpB appears to have an instrumental function in establishing the correct reading frame during trans-translation.
Domain III is essential for establishing the proper reading frame, so the mutations could induce the ribosome to initiate out-of-frame.
Previous studies suggest that several nucleotides immediately upstream of the resume condon are important for establishing the correct reading frame (Williams et al, 1999; Lee et al, 2001; Miller et al, 2008).
The study was conducted on the 54 genes in which both a full-length EST contig and a full-length genomic sequence could be established for the entire open reading frame (see Additional file 4: Figure S3).
