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To establish randomly placed, single-copy landing sites, we used the well-characterized Sleeping Beauty transposon system 100x (SB100x) (Grabher and Wittbrodt, 2007; Ivics et al., 1997; Mátés et al., 2009).
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The area of the study site is about 10,000 m2 (100 m × 100 m), and three plots (10 m × 10 m) were established randomly.
The communication requirement could be further cut down through a protocol where inter-node connection for message exchange is established randomly and each node transmits its local data to only one remote sensor at each time step [155].
In each elevation range (block) and land use types three sample plots (20 m × 20 m) were established randomly and five small soil pits (from four corners and at the center) were opened in an 'X' design.
The number of plots was estimated from reconnaissance survey prior to the main study, whereby, seven plots were established randomly in order to obtain the coefficient of variation (CV).
The set of subsystems from whom a given subsystem receives fluxes was also decided randomly, (being thus established randomly the flux architecture of the net) as well as the parameters associated to the flux integration functions.
A fourth sole population was established from randomly selected juveniles from every stock to constitute the ungraded control subpopulation (mean weight of 155.1 ± 57.8 g).
To characterize and compare the canopy composition and forest structure of pure and mixed stands, we established 10 randomly located, independent plots for each forest type and geographical location (N = 3 × 2 × 10 = 60).
The null distribution was established by randomly selecting genes.
The populations were established by randomly choosing N individuals with replacement out of the H founders.
In early May 2009, we established three randomly selected 60-m line transects through a patch of C. candidum (n > 250).
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