Sentence examples for establish double from inspiring English sources

However, we were unable to establish double mutant strains of ph505 and another gene mutant.

We also tried to establish double silencing of Prx I and Prx II in HCC cells, but we found that double depletion of Prx I and Prx II could trigger cell death so as that a stable Prx I/II double-deleted cell line could not be established, which was similar to the effect of complete elimination of Prx I.

We also applied CD tagging to establish double-labeled reporter cells, in which one protein is tagged with YFP and the other with a red fluorophore, mCherry.

Efficient methods to establish doubled haploid lines are essential for researchers to map genes of agronomically important traits as well as for breeders to shorten the time of the breeding process required to produce new hybrids and homozygous varieties.

Moreover, the electric field effect is discussed along with the recently established double reference method in the DFT framework.

Therefore, the scope of the present review is to provide a comprehensive account of the architectures built up from these ligands rationalizing them through a conscientious analysis of their coordination modes in which the preponderance for establishing double chelating five-member rings holds a central role.

CoaE activity was assayed using two different coupled assays; the established double coupled pyruvate kinase/lactate dehydrogenase (PK/LDH) and a single-coupled α-ketoglutarate dehydrogenase (KDH) assay [16].

CoaE activity was assayed using two different coupled assays; the established double coupled pyruvate kinase/lactate dehydrogenase (PK/LDH) (24) and a single-coupled α-ketoglutarate dehydrogenase (KDH) assay.

This complex interaction can be illustrated by a well established double paracrine mechanism: NHKs release IL-1 [21], [29] [30]]–[30] and or parathyroid hormone-related protein (PTHrP) [21], [31] to induce the expression of growth factors such as KGF, GM-CSF and IL-6 in HDF, which in turn regulate both NHK proliferation and differentiation [21], [30].

KB crossed a variety of mutant plants and established double and triple mutant lines.

However, the lack of established double labeling has made it difficult to ascertain the identities of the metamorphosing epithelial cells.