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Thirty-minute spray tests were used to establish concentrations at which overchallenges of the contact-kill surfaces occurred.
Initial experiments were directed to establish concentrations of proteasome inhibitor not associated with cell death within the time frame of our experiments, since cell loss might be accompanied by possible secondary redox and other signaling events.
UV spectroscopy (NanoDrop ND-1000, Thermo Scientific, USA) was used to confirm purity of the RNA samples and establish concentrations, whereas RNA integrity was assessed by agarose gel electrophoresis and ethidium bromide staining.
In other experiments in which, prior to IL-1 stimulation, time was allowed for the IL-1Ra producing cells to establish concentrations equivalent to that of the recombinant, the inhibitory curve was shifted slightly to the left, indicating a slight increase in the effectiveness of IL-1Ra when provided as a transgene product.
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Parallel experiments were performed with [H]LCV to establish concentration-dependent accumulations of [H]folates.
Although substantial evidence exists on molecular and physiologic impacts, the evidence needed to establish concentration-response functions is somewhat limited.
In addition, while diffusion will establish concentration gradients, as a further simplification compartments are considered to have uniform [O2].
It therefore became necessary to establish concentration ranges associated with cytotoxicity, with the aim of excluding data points confounded by cytotoxicity from concentration response analyses.
The effect of each mutation on the potency of IHERG blockade by doxepin was determined using the same protocol as was used to establish concentration-dependence of WT IHERG blockade (Fig. 1).
Experiments such as those illustrated in Figures 2(a), 2(b), and 2(c), but without CO2 measurements, were repeated with 50 and 200 μM hesperetin and naringenin and with 200 μM hesperidin to establish concentration dependences for the effects.
Comparing the lead concentrations in the collected sources with the established concentrations of concern discarded 0 to nine sources (median = 4) per child.
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