Sentence examples for establish a normalization from inspiring English sources
To control for inter-experimental variation in CD3 zeta-chain mean fluorescence, cells from a single donation, from a healthy donor, were cryopreserved in aliquots and one aliquot was thawed with every experiment to establish a normalization factor.
This case study can serve as a starting point to establish a normalization framework for the use of gene expression data in comparative physiology studies and gives hints for the use of the cellular stress response as a biomarker to study environmental stress and stress adaptation under changing environmental conditions.
Thus, in order to establish an array normalization probe set, 1000 highly conserved genes were selected using in silico analysis of the nine completely sequenced SA genomes used to generate the array probes.
We first establish a definition of a correct normalization when inference is performed on individual probes (e.g. when there is one probe per gene).
Additionally, a multiple response based on the normalization of the concentrations by the highest values was built to establish a compromise condition between the two analytes.
To establish a Physcomitrella cDNA library representing most genes expressed during vegetative growth before the onset of differentiation, RNA was extracted from protonemata cultured for different time periods in liquid culture, and a cDNA library in plasmid vectors was established after normalization to decrease redundancy [ 16].
Establish an actual budget.
The normalization established an equivalence of 1.56 transcripts in O. sativa for one transcript in A. thaliana.
