Using molecular biology approaches, we demonstrate that ectopic expression of META1 and site-directed mutagenesis of selected residues in the predicted META1 cavity do affect secretory processes in Leishmania, thus establishing a function for this poorly characterized protein as well as identifying a functional domain within the molecule.
"It's clear beyond a shadow of a doubt that they've established a function" for PrP, says Neil Cashman, a neuroscientist at the University of British Columbia in Vancouver, Canada.
Some key functions where the authors establish a role for Ki-67 are less completely explored.
Several works have been able to establish a concrete function for some miRNAs related to MS. For instance, Du and colleagues found that miR-326 was upregulated specifically in MS patients with relapse (not in remitting patients) and more prominently in CD4+ T cells compared to CD8+ T cells and non-T cells.
These data provide insight into a mechanism by which innate immune signals can modify cellular defenses and establish a new function for the NLRP3-inflammasome and caspase-1 in host defense.
To the best of our knowledge, our analysis is the first to establish a stabilizing function for such elements by a systematic approach.
Our findings establish a new function for Cdc48/p97 in RAD.
Future studies will be required to establish a causative function for Creb3l1 in vascular integrity disorders.
Although this experiment did not establish a replication function for Hat1p at these two origins, it nevertheless showed that plasmid loss occurs below a threshold level of core histone acetylation.
As we first implicated LPA as a potential mediator in breast tumourigenesis by studies of breast cancer cell lines (Xu et al, 1995), many subsequent studies have combined to establish an important function for the ATX LPA axis in the development and progression of breast cancer.
Moreover, we show that LPS challenge suppresses the expression of genes involved in metabolism and we establish a critical role for Tyk2 function in this downregulation.
