In an effort to easily transfer genes from Gateway-based Arabidopsis cDNA libraries to the L. lactis expression vector pNZ8148, we first established a cloning strategy compatible with Gateway entry vectors.
We have established a cloning system based on the NL4-3 virus that allows the exchange of the gp120 V3 loop.
We have neglected disadvantageous alterations since in large tumors, cells carrying such alterations are likely unable to establish a surviving clone, but go extinct due to their deleterious characteristics.
The drug shown in panels a and b does significantly decrease the population of stem cells, and therefore any resistant stem cell that arises will not be inhibited by the density constraint mechanism and be able to establish a resistant clone.
Five separate transformants were grown for each construct and compared for growth and expression levels in order to establish a representative clone for upscale culturing.
The aim of the present study is to establish a bacterial clone capable of secreting an integrin αv β3 targeting probe with bioluminescent and fluorescent activities, and to verify its specific targeting and optical activities using molecular imaging.
We established a stable clone from H460 cells by expressing the constitutively active STAT3 (STAT3C) gene.
For example, in 16 mosaics, only AB, one of the daughters of the zygote, had established a positive clone.
In contrast, there were no reciprocal mosaics in which P1, but not AB, had established a positive clone.
We established a stable clone of PC12s with impaired autophagic degradation due to overexpression of functionally inactive Atg4 (dominant negative (DN -Atg4).
