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But Liu believes that the relationship is essentially preparation for when large online travel giants enter the travel activity space.
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But for much of this century they were essentially a preparation for serious adult roles: baby dolls and toy kitchens for girls, erector sets, toy trucks and train sets for boys.Enter, in 1960, Mattel's Barbie: not a baby, but a babe.
The protocol for analysis of sequence variation is described in Additional file 8. Essentially, after preparation of multiple alignments, the variation among sequences was quantified and characterised using phylogenetic estimation.
Although plasma samples were analyzed essentially without sample preparation, we optimized the preparation method to decrease the extent of the matrix effect and to obtain the maximum signal intensity.
The removal takes place in essentially the same preparation as described above for balloon placement, with the same fetal and maternal medications.
The main advantage of this procedure is that it requires essentially no specimen preparation and allows continuous measurements after TS deposition.
Preparation essentially within the late July to mid-September 1940 time frame was made possible by extensive use of Ordnance and British Geological Survey publications, information readily available, rather than data from other sources.
Library preparation essentially followed the method described in [ 17].
The glands were imaged immediately and then fixed and processed for whole mount preparation essentially as previously described [ 17].
The chromosomal location and chimerism of YAC clones were determined by FISH analyses, using B1-PCR of YAC DNA for probe preparation essentially as described [ 49].
As a result, PCT has emerged as a powerful technique to investigate such materials at the submicrometer scale and three-dimensional perspective with essentially no sample preparation or perturbation.
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