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Until 2009, the search and identification of inks were essentially performed manually.
This was essentially performed as previously described [15].
All histological procedures were essentially performed as described previously [17].
Quantitative RT-PCR was essentially performed as described previously [46].
Electromobility shift assays were essentially performed as previously described [26].
Coprecipitations were essentially performed as described [18], [22], [27], [43].
In vivo ubiquitination assays were essentially performed as described [8].
ChIP was essentially performed as described in [56].
cDNA synthesis and qRT-PCR were essentially performed as described [39].
Both polyclonal and monoclonal antibodies were generated and they essentially performed equivalently.
Immunoblotting procedures and immunoprecipitation experiments were essentially performed as described [33], [42].
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