After differentiation into definitive endoderm, foregut endoderm was differentiated, essentially as described (Green et al., 2011).
It is important to note that until this stage, the intestinal epithelium consists of essentially only the differentiated larval cells.
ES cell-derived glutamatergic neurons were differentiated essentially as we have previously described with a minor modification (see below).
ESCs were differentiated essentially as described previously (Wilkinson et al., 2013), and treated as described in Fig. 2A and Fig. 3A.
Cytokeratin 20 (CK20) is an intermediate filament protein whose presence is essentially restricted to differentiated cells from gastric and intestinal epithelium and urothelium [ 29].
What essentially is missing in differentiated services-based QoS deployments is their adaptability in medium access and transmission for different traffic types according to their traffic characteristics.
The fact that Oct4+/− cultures not only lack cells expressing the highest Oct4 concentrations present in Oct4+/+ cultures but are also essentially devoid of the differentiated Oct4-negative cells that are readily detectable in WT cultures is of no small interest here.
Such a reduction in the vertically differentiated variety is essentially equivalent to being variety averse, discussed in a number of empirical studies (e.g., Levav et al. [2010]; Kaiser [2011]).
In addition, the relative abundances of different histone H2B variants remained essentially unchanged between proliferating and differentiated cells.
These differentiated genomes behave essentially as meiotically independent sub-genomes (homoeologues) that almost exclusively form bivalents between chromosomes of the same sub-genome, resulting in essentially disomic inheritance.
Despite the physiological importance of adipocytes derived from human or mouse skeletal muscle, characterization of these terminally differentiated cells is essentially limited to the expression of adipogenic markers.
