WFA was found to be localized to the target and adjacent tissues with 12 16 ng WFA/g tissue, with essentially no detectable WFA in distant tissues.
Primer extension analysis revealed that essentially no detectable full-length product was observed in the presence of the highest amount of DNA oligonucleotide, when LNA-AUG1 was transfected into cells.
Moreover, four negative control ChIPs with a pre-immune rabbit IgG (Millipore, cat# PP64B; http://www.millipore.com) only precipitated minute amounts of DNA; these were estimated by spectrophotometry to represent ∼0.3% of input DNA and were essentially not detectable by qPCR (data not shown).
Activation of c-Myc in the epidermis, however, is associated with essentially no detectable apoptosis when examined by co-staining with the specific suprabasal keratinocyte marker keratin 1 together with TUNEL.
CK7, a cytokeratin commonly associated with gynecological malignancies, was essentially not detectable in the PMP-1 model, with the exception of slight membranous staining in two of the murine passages (<10% of tumor cells).
Following 24-h growth in LB medium in the absence of added FeCl3 or chromate, the Δ so2426 mutant (A630, 0.739 ± 0.02) exhibited essentially no detectable siderophore production over that of the cell-free control (A630, 0.726 ± 0.005), whereas wild-type MR-1 cultures (A630, 0.108 ± 0.06) produced approximately 7-fold more siderophore than the Δ so2426 mutant.
While ChR2(H134remainedned fully functional for 24 h, and showed half maximal activity even after 72 h, ChR2(C128S) was surprisingly susceptible to ATR-deprivation: Already after 4 h, functionality was reduced by ∼46%, and essentially non-detectable after 48 h (0.5±0.3% contraction; Fig. S6).
In prostate cancer samples, Id4 expression was clearly dependent on Id4 promoter hypermethylation: Id4 expression significantly decreased by 76 and 222-fold (essentially un-detectable) in partially methylated and completely methylated prostate cancer samples, respectively (Fig. 5).
By over-producing the A. bisulcatus enzyme SMT in A. thaliana, we have introduced a novel biosynthetic ability that has increased the concentration of MeSeCys and its functional derivative γGluMeSeCys, from essentially non-detectable levels in the leaves of wild-type A. thaliana up to 3.9 μmol g-1 dry weight in shoots.
Surprisingly, addition of an anti-VEGF antibody, which neutralized essentially all the detectable extracellular VEGF (Fig. S2E), had little impact on VEGFR2 upregulation in response to MET disruption in H441 cells (Fig. 2K).
