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Essentially, cells were fixed in 3% paraformaldehyde and permeabilised with 0.1% Triton-X100/PBS.
Essentially, cells were washed once in ice cold PBS before scraping and collection by centrifugation.
Cells with progenitor-like self-renewing properties dominated in low-grade gliomas and neuro-glial tumors, whereas high-grade gliomas yielded essentially cells with extended self-renewal properties (Figure 1 and Figure S3).
Essentially, cells cultured on glass coverslips were washed with PBS, fixed with cold methanol, and permeabilized with 0.2% Triton X-100 in PBS.
Essentially, cells were trypsinised, resuspended in complete DMEM, washed twice with PBS, incubated in 10 μM DHE dissolved in pre-warmed PBS for 15 min and analysed by flow cytometry.
Similar(54)
VEGF189 and VEGF206 are poorly secreted and are essentially cell associated although their peptide signal sequence is identical to that found in VEGF121 and VEGF165 (Houck et al. 1991).
Smartphones are essentially cell phones with advanced connectivity and computing capability.
These results demonstrate that the dendritic changes observed with stable ablation of miR-9 are essentially cell autonomous and not caused by changes of cell extrinsic factors.
This indicates that effects found in miR-9-SpNestin-Cre mice are essentially cell-intrinsic and that miR-9 exerts its proactive function in modeling dendritic arbors as a key player of neuronal genetic programs controlling dendrite development.
Essentially all cells in the body are target cells of triiodothyronine.
Because viral oncogenes essentially turn cells into a tumor, they tend to change the cells' characteristics.
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CEO of Professional Science Editing for Scientists @ prosciediting.com