For the essential temperature-sensitive mutants, all growth was conducted at 23°.
In summary, the query strain DTK1189 2b was mated to a MATa essential temperature-sensitive (ts) strain set containing 455 ts genes (from Dr. Charles Boone, University of Toronto) (Li et al. 2011) and incubated at RT for 2 d.
This suggests that CK1ε is not essential for temperature compensation, likely due to temperature-compensated activity of CK1δ.
Each possessed a unique color (red, white, yellow and black) and an essential character, temperature and taste.
The full solution is essential for temperature transient analysis in smart wells – a monitoring approach of great potential, but in early development stage.
The solution is essential for temperature transient analysis in smart wells a monitoring approach of great potential, but in early development stage.
Here, it is essential that temperature, extraction time, and stirring are identical among samples and standard solutions, which makes an autosampler with SPME device and agitator (well-defined shaking and heating) indispensable.
We suggest that whereas UCP1 in iWAT plays an important role in protection against obesity, UCP1 in iBAT is essential for temperature adaptation.
We find that many genes previously identified as essential are temperature sensitive and also identify a number of genes that, when disrupted, confer a growth advantage at elevated temperatures.
Since ESA1 is essential, the temperature-sensitive esa1 mutant was grown at nonpermissive temperatures and samples were prepared.
Mr. Mossessian, who is working on the development's center square, says: "The overall plan has been to keep the original street patterns of narrow alleys that zigzag between densely packed houses which faced north so that people could walk in the shade as much as possible -- essential when temperatures reach 55 degrees" Celsius (131 degrees Fahrenheit).
