Sentence examples for essential modified from inspiring English sources

The cells were grown in Essential Modified Eagle's Medium (EMEM) supplemented with 4% fetal bovine serum, 2 mM of L-glutamine, 50 µg/ml penicillin and 50 µg/ml streptomycin.

Detailed growth measurements were analyzed to reveal effects on ~5,500 different haploid strains that have either non-essential genes deleted or essential genes modified to generate unstable transcripts.

The MCs were plated on 100-mm plastic dishes (Nunc) that were maintained in B medium (a 3∶1 mixture of minimal essential medium/F12 modified with trace elements) supplemented with 1 mM glutamine, penicillin at 100 units/ml, streptomycin at 100 µg/ml, and 10% fetal calf serum (Irvine Scientific).

The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies).

Indeed, steady state levels of the essential proteins modified with the GFP-cODC1-TDegF tag were reduced in comparison with the corresponding GFP-tagged proteins (data not shown).

HeLa S3 were maintained between 5-10 × 105 cells/ml throughout the experiment and, before the time course, were grown in minimum essential Joklik modified media (Sigma Aldrich, St . Louis MO, USA) as previously described [ 22].

For virus production, about 5x107 HeLa-H1 cells (Flow laboratories, Irvine, Scotland) were seeded per 1 L minimum essential medium modified for suspension (Sigma, Vienna, Austria), containing 7% heat inactivated horse serum, 100 U/ml penicillin and 100 µg/ml streptomycin (all from Gibco, Rockville, MD) and grown at 25 rpm in a 2 L spinner flask (Bellco, Vineland, NJ) at 37oC for 4 days.

The understanding of pathways and crucial enzymes is essential to modify microalgal strains.

The designation of a gene or regulatory element within this scheme, i.e. an essential gene, a non-essential fitness modifying gene, a fitness neutral gene, a tumor suppressor/gatekeeper gene, an oncogene or a caretaker gene is fundamental for lineage selection effects through changes of physical and functional genome redundancy.

Understanding these experiences is essential to modifying high-risk behaviors while supporting patients through their rehabilitation.

In order to conserve and recycle water, it was firstly deemed essential to modify the operating regime of the ISLSS.