WT HBE41o- and CFBE41o- (ΔF508 homozygous) cells were cultured in MEM (Minimum Essential Medium) supplemented with 10% FCS (Fetal Calf Serum), 2 mM glutamine and 1% penicillin/streptomycin.
KBM-5 cells were maintained in Iscove's modified Dulbecco's medium supplemented with 15% FBS, A293 cells were maintained in minimum essential medium supplemented with 10% FBS, and H1299 cells were maintained in RPMI 1640 supplemented with 10% FBS.
Briefly, LLC cells were cultured in minimum essential medium supplemented with 10% foetal bovine serum.
Briefly, the cells were cultured in Eagle's minimum essential medium supplemented with 5% of human platelet lysate (EMD Millipore), 100 μg/mL, 100 U/mL penicillin, streptomycin, and 250 ng/mL amphotericin B (Cellgro) at 37 °C with 5% CO2.
The cells were maintained in Minimal Essential Medium supplemented with 10% FBS, penicillin (100 U/ml), and streptomycin (100 μg/ml) in a humidified atmosphere of 50 μg/ml CO2 at 37 °C.
HEK cell lines transfected with 482R, 482G and 482T plasmids were grown in the minimum essential medium supplemented with 10% FBS, 50 IU/ml penicillin, 50 μg/ml streptomycin, 4 mM L-glutamine and 100 nM Geneticin.
It was cultured in RPMI 1640 medium and minimal essential medium supplemented with 10%% fetal calf serum, 100 U/ml penicillin, and 100 μg/ml streptomycin, 4 mM l-glutamine under 5%% CO2, and 95%% humidified atmosphere at 37 °C.
MDCK cells were maintained in Eagle's minimum essential medium supplemented with 5% FBS.
P19CL6 cells were grown in Dulbecco-Modified Minimal Essential Medium supplemented with 10% fetal bovine serum.
A498 cells were cultured in Minimum Essential Medium supplemented with 10% fetal calf serum and non-essential amino acids.
In brief, cell were plated in chamber well slides and cultured in Dulbecco's Minimum Essential Medium supplemented with 10% fetal bovine serum until confluent.
