Exact(9)
Molecular docking study between the most active compound and NA suggested that hydrogen bonds, hydrophobic and electrostatic interactions were closely related to NA inhibitory activity, 5-OH and 7-OH may be essential for this activity.
The article (with Nico Adams from CSIRO) "Semantic Physical Science - Making Physical Science Data Accessible and Useful" shows why semantics are essential for this activity.
The ATPase function appears essential for this activity, because mutating an essential lysine residue in the ATPase domain abrogates this function.
Together, these data suggest that the conserved region surrounding SIM1 is sufficient to promote modification of a linked consensus sumoylation site, and that the SIM is essential for this activity.
As shown for other homologues, mATX3, specifically its josephin domain (mATX3:jos), is able to cleave either K48 or K63-linked polyubiquitin chains, the cysteine 14 residue being essential for this activity (Figure S2).
When the expression of the DNASE2 gene was suppressed by siRNA-mediated knockdown, the predominant acid DNase band was clearly diminished as compared to skin models treated with control siRNA, suggesting that DNase 2 was essential for this activity (Figure 4A, B).
Similar(51)
The transcription factor AP1 is essential for the activity of this promoter, a finding mainly based on the observation that mutation of the corresponding binding sites within the HPV18-LCR completely abolishes P105 promoter activity in human keratinocytes [ 14, 16].
The cysteine of this triad is essential for the activity of Cifs as mutation of this residue leads to loss of the cell cycle arrest phenotype in cell-based assays [8], [14].
Klothos belong to a family of enzymes that cut sugar chains1, but not all of the amino-acid residues essential for this enzymatic activity are found in the klothos.
Five recognition sites appear to be essential for this dual activity: two hydrogen bond acceptors, an anionic site, a hydrophobic group and an aromatic ring.
Lysine 10, which lies within a putative sumoylation motif, was essential for this inhibitory activity.
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