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Furthermore, no genes for gyrase (needed to relax the positive supercoils generated by the replication process), ligase (essential for joining the DNA fragments in the lagging strand), and RNAse HI (needed to eliminate the RNA primer) have been found.
The absence of stalled transcription products when reading through click-linked DNA with T7 RNA polymerase in vitro further supports this hypothesis.[ 19] Our results indicate that a phosphodiester linker is not essential for joining oligonucleotides for gene synthesis and open up the possibility of replacing enzymatic ligation with highly efficient chemical reactions.
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These two successive GTP hydrolysis events, and the simultaneous release of eIF3, are essential for the joining of the 60S subunit.
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