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Therefore, to determine whether Fam40b is essential for differentiation of ESCs toward different cell types, we differentiated cells in 'hanging drops' using KD ESCs in comparison with the Scr ESCs and WT ESCs.
By blocking cell division from the gastrula stage, we show that cell proliferation is not essential for differentiation of cardiomyocytes, and the resulting heart remains essentially normal in size, as determined by the expression of markers of cardiac differentiation.
Cells of the osteoblast lineage also provide factors essential for differentiation of osteoclasts (bone-resorbing cells).
Inactivation of Notch1 did not affect the development of pDCs, suggesting that this receptor is not essential for differentiation of this cell subtype (Radtke et al., 2000; Lewis et al., 2011; Ferrero et al., 2002).
Maintaining normal cell morphology may also be essential for differentiation.
Control of p53 by Shh is also essential for differentiation of some amacrine cells and photoreceptors.
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Thus, MafB is an essential factor for differentiation or functional maintenance of myeloid cells.
This gene plays a crucial role in muscular differentiation and its activation is essential for myogenic differentiation [ 45].
However, it is still controversial whether increased ROS is essential for adipocyte differentiation or it is a byproduct of the differentiation process.
Id2 also promotes differentiation in MEC cultures, indicating Id2 is essential for the differentiation of the mammary epithelium [ 63].
Differential control of gene expression is essential for tissue differentiation and development.
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