Gp6 is essential at early steps of DNA packaging and for DNA translocation to the capsid interior, it affects the efficiency of DNA packaging, it is a central component of the headful sensor that determines the size of the packaged DNA molecule, and is essential for closure of the portal pore by the head completion proteins to prevent exit of the DNA encapsidated.
Moreover, Atg12-Atg5-Atg16L is functionally required to conjugate LC3-I to phosphoethanolamine [ 20], which is essential for closure of the expanding autophagosomal membrane [ 21].
These results suggest that the Asn207-Arg91 hydrogen bond is essential for full closure of the 200s flap, and complete flap closure is necessary for full catalytic efficiency.
This is in accordance with the recent observation that SLAC1 is essential for stomatal closure in response to ozone, ABA, Ca2+ ions, and H2O2 in Arabidopsis [31].
It is noteworthy that recently, SLAC1, which represents the slow type anion channel of guard cells [28], [29], was shown to be essential for stomatal closure in response to O3, Ca2+ and H2O2 [31].
Recently, an anion channel SLAC1 [28], [29], [30], [31], [31] was shown to be essential for stomatal closure in response to ozone, Ca2+ ions and H2O2 in Arabidopsis [31], suggesting that anion efflux could be induced in guard cells by O3 through an increase in anion channel activity.
The FERM-binding domain motif (FBM) of Crb is essential for dorsal closure (DC).
This vitamin is essential for timely closure of the cranial bones.
In the subsection "The FBM of Crb is essential for dorsal closure", first paragraph: The authors conclude the FERM binding domain of crb is responsible for the effects that they see because a point mutation (Y10A) in the FBD fails to rescue crb mutant function.
In the subsection "The FBM of Crb is essential for dorsal closure", fifth paragraph: The authors should show on the schematic in Figure 1 the orientation of the cross sections shown in Figure 1 panels M, N and O. Neither the text, nor the figure legend adequately describes the orientation of the section, and how it was obtained.
In the subsection "The FBM of Crb is essential for dorsal closure", the authors state: "Moreover, in foscrb and foscrb Y10F lacking zygotic yrt DC fails only after GB retraction, which is qualitatively different from the phenotype of foscrb Y10A or foscrb Y10A embryos lacking zygotic yrt (Video 4)".
