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The Corynebacterium glutamicum ortholog (NCgl0187) could not be deleted by Meniche and colleagues [9] and the M. tuberculosis ortholog (Rv3790) is thought to be essential based on a lack of insertions in transposon mutagenesis experiments [14], however, in both cases, direct experimental evidence of essentiality is lacking.
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In Table 4 and 5, there are ~30% of genes in the co-targets set are essential based on the essentiality information retrieved from TB [ 70] and DEG v5.0 database [ 71].
The four Arabidopsis poly(A) polymerase genes were found to be essential, based on genetic analysis of T-DNA insertion mutants.
Secondly, if it were a priority, i.e. it was considered as essential based on the disease status or age of household members, particularly spouse and young children, the answer would again be affirmative.
We extracted a list of human proteins that are likely to be essential based on the fact that knockouts of their orthologs in mice are annotated in the Mouse Genome Database as producing pre-, peri- and post-natal lethal phenotypes.
While much digital ink has been spilled on the topic of how to unleash innovation in organizations, here are three ingredients of innovation that I've observed are essential based on my experiences.
The criticality of offshore drilling safety appears more essential based on the stated statistics by the head of the International Energy Agency of the OECD.
This could explain the result with the STM strains of the argS gene since the arginyl-tRNA synthetase appears to be essential based on our failure to obtain viable argS deletion mutants.
Finally, we examined which genes/reactions are predicted as essential based on the enterobacteria ancestral core metabolic model.
An additional 10 genes were found by SA to increase the number of reactions necessary to produce biomass and deemed essential based on this criterion.
This cut-off was chosen empirically to minimize the number of false positive genes that were considered unlikely to be essential based on their annotation or prior experimental evidence, whilst retaining most known essential genes.
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