"The conversations were sometimes lengthy and Poole especially expressed great concern at the possibility McBride was right," Woodhouse said.
This may lead to many markers, especially those expressed at low levels, not being detected [ 15].
These novel sporozoite expressed genes, especially those expressed at high levels in salivary gland sporozoites, are likely to play a role in Plasmodium infectivity in the mammalian host.
However, it is still difficult to identify M-cell-specific molecules, especially those expressed at low levels, because the M cells constitute only ∼5 10% of the FAE cells.
In a recent study, stably expressed QTLs for yield and associated traits at different N levels, especially those expressed at low N, were reported in recombinant inbred line populations (Tong et al. 2011).
There is thus an increased probability that in the presence of TCDD, the stochastic fluctuations in the levels of key transcriptional repressors, especially those expressed at low levels in the plasma cell (i.e., Pax5 and Bcl-6), may swing the system across the fate-dividing boundary to the B cell state.
It is likely that this trend relates to the fact that small variations in mRNA levels (<2-fold) can be accurately detected by qRT-PCR, while the smaller dynamic range of microarrays means that the same changes may not be accurately reflected by microarray expression scores, especially for genes expressed at low levels (<1.5 pM or approximately 3.5 copies/cell) [ 18, 19].
This within-sample variance is a significant source of uncertainty in oligonucleotide arrays, especially for genes expressed at low levels.
In a related study [ 12], we showed that, for Agilent microRNA data, the use of the RMA algorithm without background correction reduced signal variability, especially for genes expressed at low intensity.
Taken together, our previous research using SSH technology has provided important clues for understanding the formation of the mutation trait in precocious trifoliate orange; however, the transcriptional information from SSH, especially for genes expressed at low levels, is rather limited.
