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Esophagography revealed that stenotic change of the ESD site was very mild.
Follow-up EGD performed 2 months later revealed no local recurrences at the ESD site, but three new erosions appeared on the gastric angle and antrum (Fig. 2a, b).
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We hypothesized that the long-idle ES site, with higher initial soil C and N stocks, would have higher GHG emissions following conversion compared to the recently-idle RH site, but that this would be balanced in part by greater SRWC productivity at the ES site.
ES site sequences analysis identified some over-represented sequence motifs shared by subsets of ES sites.
Motif 1 was 21 bp long and shared by 85 ES sites; motif 2 was 15 bp long and shared by 43 ES sites; and motif 3 was 11 bp long and shared by 69 ES sites.
However, not all ES sites (892) were covered by the 3 motifs, suggesting additional motifs that could not be identified by this method.
Towards understanding how PCFS4 might gain its target specificity, we identified the genome-wide PCFS4-TAP ES sites that were specifically concentrated on the genic regions (Table 1).
892 ES sites were identified with the following criteria: Log2 Fold Change ≥ 5; p-value ≤ 0.001; and the false discovery rate (FDR) = 0.02 (Additional file 2: Table S1).
Most importantly, the ES sites on these genes were often associated with the positions where the alternative processing or alternative transcription initiation occurred (Additional file 1: Figure S3).
To verify the enrichment of PCFS4-TAP on the identified ES, we performed a real-time PCR (qPCR) analysis following the ChIP for 9 randomly selected ES sites, which cover the entire ES list with p-value ranking from low to high (Additional file 2: Table S1).
MT: investigated, designed, and collected the data at the Dar es Salaam site.
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