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By depletion of Tet1 and Tet2 in ESCs, it was shown that Tet1 and Tet2 indeed played distinct roles in DNA demethylation.
With the technology of reprogramming somatic cells by introducing defined transcription factors that enables the generation of "induced pluripotent stem cells (iPSCs)" with pluripotency comparable to that of embryonic stem cells (ESCs), it has become possible to use this technology to produce various cells and tissues that have been difficult to obtain from living bodies.
Although we cannot answer the question of whether iPSCs are truly functionally equivalent to ESCs, it seems increasingly obvious that there exists more than one state of pluripotency.
Sox2 is not "bivalent" in ESCs, it is actually expressed.
However, as the EST uses murine ESCs, it may not reliably detect human-specific toxicity (Krewski et al. 2010).
In mouse embryonic stem cells (ESCs), it allows for gene mutation and the simultaneous generation of the corresponding mutant mice.
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Rex-1 is involved in maintaining an undifferentiated state of ESCs and it is also expressed in bone marrow MSCs, but its function in the dental follicle is unclear.
CSPG does not stimulate the differentiation of ESCs, thus it is likely to enhance the survival of ESC-derived NSCs similar to its effect on brain-derived NSCs.
Glis1 is highly expressed in unfertilized eggs and zygotes but not in ESCs; thus, it remains to be determined if other maternally expressed genes could similarly reinitiate pluripotency.
In line with this characteristic, c-Myc is abundant in self-renewing ESCs, whereas it undergoes downregulation in response to induction of differentiation.
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