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We discuss our findings in connection with specific features of the asymmetric Rho structure yet argue that a simple RNA "escort" model is insufficient to account for all experimental evidence.
As a result, the two-subunit escort model predicts that SpoIIIE should introduce one supercoil for every ∼80 bp of DNA translocated.
(b ) Diagram of the SpoIIIE hexamer illustrating how the two-subunit DNA escort model enables the motor to bypass an ATPγS-bound subunit (red).
Finally, we propose a two-subunit DNA escort model that can rationalize all these data and that correctly predicts the degree of supercoiling introduced by SpoIIIE during translocation.
In the two-subunit DNA escort model, the DNA is handed over from one subunit to the next and, for each 2-bp step, the motor-DNA contacts rotate clockwise around the ring by 60°.
An escort model for FtsK translocation can accommodate our observation of normal velocity yet reduced ability to displace roadblocks when two catalytically inactive subunits are introduced into a hexamer.
Similar(51)
To be consistent with the results of the stepping-stone insert experiments, our two-subunit translocation-escort model requires that subunits fire in a well- defined sequential fashion (A, B, C, D, E, F, A, B, C etc) and a subunit cannot fire multiple times in a row or out of order.
The Shariah police loaded the competition's trophies into a bag and escorted models out of the building.
In 2006, after moving to New York with his musical Taboo, he was sentenced to sweep the streets of New York for possession, and in 2009 was jailed in Britain after a bizarre case involving the false imprisonment of a 29-year-old Norwegian male escort and model, Audun Carlsen.
Actress, escort and model Anastasia Vashukevich ― who goes by the name Nastya Rybka ― took the videos and posted them online.
A travelling business man from Chillicothe, Ohio, escorts a model around N.Y.
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CEO of Professional Science Editing for Scientists @ prosciediting.com