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*Each guideline was checked for 16 recommendations and compared to the European guideline (ESC) of 1997 † see Figure 1.
Importantly, ESC of human and mouse origin differed in their requirements for Cdk2; being critically important for the maintenance of pluripotency in human cells.
Unlike previous reports [ 27], we examined very young embryos (0 2 h), before cellular blastoderm and the mid-blastula transition, while all the cells are still pluripotent (a situation more closely related to the ESC of mammals).
Three samples of populations of SSEA-4-positive putative ovarian stem cells (OSC), isolated by FACS from three different ovarian cell cultures in three different patients, were analyzed by microarrays in comparison with the positive control-three samples of hESCs (ESC) of H1 cell line (WiCell, WI, USA) and the negative control-three samples of human adult (dermal) fibroblasts (FB).
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When we evaluated the LIF responsiveness of rat ESCs on expression of Socs3 and Egr1, we found a similar pattern to that of mouse npm-ESCs.
Yet other functional modules were not conserved; instead, they were significantly up-regulated in ESCs of either species, suggestive of species-specific regulation.
As shown in Fig. 3A, siRNA-mediated knockdown significantly depleted ESCs of endogenous Cobra1 protein.
In contrast with the ESCs of Figure 1, these cells originated from a specialized adult organ.
Our data support these experimental observations and further suggest that these mechanisms are possibly conserved among ESCs of different species.
Four ESCs of each clone were simultaneously injected per blastocyst then transferred into pseudopregnant females.
We established three male ESCs of each type from these six strains using 2iLIF with MEF.
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