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It was also observed that many genes containing these sequencing errors were annotated in the published closed genome as encoding proteins containing frameshift mutations (18 instances) or were annotated despite the coding frame containing stop codons, which if genuine, would severely hinder the organism's ability to survive.
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The adjusted P-value used here made it possible to control the Family Wise Error Rate (FWER) Microarray ESTs were annotated as described in [ 60, 71].
Genomes were annotated using the RAST annotation system performed on 9/10/12, backfilling of gaps and automatic error fixing were enabled.
Based on MS2 fragmentation patterns and exact mass determination (4-digit mass errors of < 1.6 ppm), these peaks were annotated as desulfoBGLS (dBGLS) and malonylated dBGLS (mdBGLS).
Sequences with terminal positions outside this window were annotated as potential deletion/extension errors.
Contigs were annotated as above.
Called SNPs were annotated with SnpEff software54.
Protein domains were annotated with InterProScan (v5.19 85.
Identified variants were annotated using Oncotator.
All the books were annotated and excerpts were provided.
Both datasets were annotated by experts.
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