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The sequencing reaction was carried out for each strand of DNA to avoid possible errors of incorporation of nucleotides by Taq polymerase.
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Again, the polymerase exhibited low levels of error-prone incorporation of dATP and to a lesser extent, dGTP, opposite to the adduct.
A high-throughput short oligonucleotide sequencing assay (HT-SOSA) was used to quantify the types and frequencies of incorporation errors produced by the human Y-family DNA polymerases at and near the 8-oxodG site.
This is prone to a greater error rate than the discrimination of incorporation versus non-incorporation [ 31].
DNA polymerase V is responsible for error-prone translesion synthesis (e.g., following UV-irradiation), which allows DNA synthesis to proceed despite a high rate of error incorporation [ 32], yet there are organisms, including V. cholerae El Tor, that apparently do not encode these functions [ 33, 34].
As there is a large gap between the appearance of the previous A (position 6), there is a higher probability of incorporation and, hence, of error.
The Dpo4 polymerase exhibited primarily an error-free incorporation of dGTP with the 5′-C XG-3′ template.
Primarily error-free incorporation of dATP was observed for the 5′-T XG-3′ template (template-primer IV); efficient extension to a full-length 18-mer product was also observed when all dNTPs were included in the reaction.
What are articles of incorporation?
There is blatant abuse of incorporation.
How? State of incorporation, of course.
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