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Errors in sequence reads generated by a Roche 454 GS-FLX sequencer are not randomly distributed along the sequence and depend on various factors.
Loytynoja, A. & Goldman ,N. Phylogeny-aware gap placement prevents errors in sequence alignment and evolutionary analysis.
Reliability of ancestral reconstructions is compromised by incorrect trees, errors in sequence alignment, incorrect substitution models, and insufficient data to estimate parameters of the substitution model.
While Shannon's theorem confirms that in close to native conformations information is transmitted with limited error probability, additional random errors in sequence (amino acid substitutions) and in structure (structural defects) trigger a decrease in communication capacity toward a Shannon limit at 0.010 bits per amino acid symbol at which communication breaks down.
These contigs may represent alleles, recent duplicates or errors in sequence data.
Thus, some of the ASEs could have been missed due to minor errors in sequence assembly.
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But there are unavoidable errors in sequencing, so to be sure the data is correct, researchers repeat the sequencing 30 times — 30 libraries' worth.
(Because of unavoidable errors in sequencing, a human genome must be analyzed a number of times to generate an accurate read). They then identified spots where his genome differed from that of the reference sequence from the Human Genome Project, and narrowed that pool down to novel variations found in genes previously linked to Charcot-Marie-Tooth or other nerve disorders.
There is, of course, much pottery, and new approaches to the study of this valuable class of artifacts have allowed the team not only to correct errors in sequences but also to suggest functions for specific areas and even rooms of buildings.
This study is an extension of our earlier work, in which we have observed that channel errors in sequences with intensive temporal activity seem to have greater impact on PSNR than on the perceived subjective quality.
Others likely arise from the presence of repeated sequence in the genome, and errors in sequencing.
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