Sentence examples for errors in nucleotide from inspiring English sources

One explanation for the complex mutation data in COS-7 and 293T cells is TLS by a highly error-prone DNA polymerase that made errors in nucleotide incorporation not only opposite the lesion but also opposite natural DNA bases near the cross-link site.

Microsatellite repeat instability (MSI) presents a notable hazard in light of its involvement in diseases such as Huntington's, myotonic dystrophy, and cancer among others (see below) and is often associated with defects in the mismatch repair (MMR) machinery, a system that specifically recognizes and repairs errors in nucleotide base pairing.

As this technology is known to generate errors in homopolymeric nucleotide tracts, SNPs and indels in these regions were filtered out.

To avoid frameshift errors in the nucleotide sequences during alignment step, the deduced amino acid sequences of the protein-coding genes were aligned and subsequently retranslated using ClustalW implemented in BioEdit v.7.1.11 (Hall 1999).

The observation that hpol η preferentially incorporated the correct nucleotide opposite each of the cross-linked bases is in stark contrast to the results of a recent study using yeast pol η, which is highly error-prone in nucleotide incorporation opposite the cross-linked G (13).

Pol ι is inherently very error-prone in nucleotide insertion, particularly opposite undamaged template bases G and T, respectively, yielding misinsertion of either dTTP or dGTP at a frequency of about 0.1 and 1 (relative to the correct nucleotide insertion), which is ascribed to its unique active site and related non-Watson Crick base pairinon-Watson Crick

But after examining blood samples from five families in the United States, Britain, South Korea and Brazil, Dr. Kaplan and Eileen Shore, a colleague, found that the rampant bone growth begins with a tiny error in the nucleotide sequence of a gene that serves as a blueprint for a protein called ACVR1.

Finally, in the course of constructing our new plasmids, we have uncovered several errors in publicly accessible nucleotide sequences for existing yeast plasmids.

This was tested under the assumptions that a) errors arising during the primer synthesis process would be randomly distributed along the primer sequence, and that b) primers containing errors in the 3' four nucleotides would bind poorly to the template DNA, thus not enable PCR amplification.

The sequence was validated and errors were rectified by comparing the final assembly with independent sequence data to further increase the accuracy of the assembly and to avoid sequence errors in the homopolymeric nucleotides.

The reported Tgo DNA polymerase from the Roche product manual has an overall error frequency of ~0.2% in nucleotide misincorporation during PCR amplification, very close to what we found in our study.