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We constructed these curves on QuORUM error-corrected data as follows.
From the error-corrected data, reads containing chloroplast genomic sequence were extracted using BLAT, as SMALT does not handle long read data, following the procedure described above for the Illumina dataset except that due to a potentially higher error rate in the PacBio data, all matches with other chloroplast genomes were retained.
This can be seen in the dependence of Type 1 error rate on the sequencing error for Fisher's exact test, whereas our model appears to have a good control of Type 1 error rates across the range of sequencing errors considered, despite both methods being applied to the same, error-corrected, data.
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Koren et al. [ 8] demonstrated that the addition of a modest amount of Illumina error-corrected PacBio data to supplement 454 sequencing data from multiple libraries resulted in a 32% increase in N50 sizes in the parrot (Melopsittacus undulatus) genome sequence assembly and other researchers have demonstrated the utility of PacBio sequence data for gap filling and genome finishing [ 23].
The Ion Torrent error-corrected subsets of data were downsampled by selecting for corrected reads greater than 3000 (for 20-25× 20-25×000 (for 12-15×) nucleotides in length, while the MiSeq error-corrected subset of dand was downsampled for reads greater than 2500 and 3250, respectively.
Thus, the average depth of coverage of the P. micrantha chloroplast genome represented by the error-corrected PacBio RS data was 320×, whilst the average depth of coverage of the Illumina reads was 9,111×.
This left us with four data sets each for the Ion Torrent and MiSeq hybrid correction to assembly 1) the full data-set, error-corrected and downsampled to 12-15× 12-15×e full data-set error-corrected, and downsampled to 20-25×, 3) the subset ofullta, error-corrected and downsampled to 12-15×, and 4) the subset, error-corrected and downsandledownsampled.
Using the time stamps of DustTrak monitors, we compiled error-corrected continuous PM2.5 and PM10 data from fixed sites and mobile monitors into a single data set, with each record representing a unique date, minute, and location.
Illumina data was error-corrected using Quake v0.3 with default parameters.
The 185x PacBio coverage and a downsampled subset (110×) were error-corrected with either 100× coverage of Ion Torrent data or 100× coverage of MiSeq data.
To reduce the effect of low-quality data, we used the QuORUM error-corrected reads (below).
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