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To reduce the influence of sampling error, we only included those females that produced at least 10 eggs in our statistical analysis.
As the final quality control, to further reduce potential impact of 454-pyrosequencing error, we only included variants that had 2 or more collapsed sequences.
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One major reason for the rather low frequency in our study compared to other studies may be that we only included prescribing errors that the dispensing pharmacists considered severe enough to necessitate a clarification contact with the prescriber.
We assume an average eye length of 23 mm, since we excluded high myopes from our study, and we only included patients with refractive errors of −5 dioptres to +5 dioptres.
We only included clones produced independently in two or more birds under the assumption that random error is unlikely to occur independently twice at the same restriction sites.
Additionally, we only included variants with two or more identical sequences in the analyses, which should ensure exclusion of random sequence errors.
We only include the differences.
We, therefore, rigorously evaluated genotyping errors and only included samples that passed our quality control (further details can be found in the supporting information).
However, this latter study only included spherical equivalent refractive errors between −6 and −12 diopters and axial lengths that exceeded 26.5 mm.
In order to reduce these types of errors, we included in our analysis only those Pfam families that map to at least 10 Incyte clones.
We used a one-sided test (only including reduction in LVM) with 85% power (β error) and 95% confidence (α error).
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