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Based on our studies of replicative, repair and specialized polymerases, it is unlikely that the observed directionality bias favoring microsatellite expansion in cells reflects the inherent error specificity of DNA polymerases.
Thus, using pol β allows us to examine mechanisms of the microsatellite mutation process in a manner that will not be biased by the inherent error specificity of the polymerase.
Conversely, when a measured value is outside of the grey zone, the necessity of performing a fluid challenge can be confirmed (value above the upper limit of the grey zone) or excluded (value below the lower limit of the grey zone) with less than 10% error (specificity and sensitivity >90%).
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f We would not necessarily expect the behavior of polymerases at the early checkpoints to account fully for the error specificities reported in Table 6.
We show for the T4L-TEM1-HIV1P set, that building networks pairing every residue (disregarding charge or any other criterion) rendered the best values for error and specificity (see Fig. 2 and Table 1).
The error in specificity, however, is typically negligible.
The mean-square prediction error, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were obtained.
Increased error, decreased specificity, and decreased AUC were found when the phase-dependent measures, λdiff and λlag, were removed (see Table 2 and Figure 5).
We extensively discussed the issue that controls in our study did not undergo colonoscopy and provided detailed model calculations to estimate the resulting error regarding specificity.
Values of AUC, which range from 0.5 (no different from random) to 1 (a perfect model), are derived from measures of sensitivity (absence of omission error) and specificity (absence of commission error).
Further, the controlled vocabulary to which the gene product is curated can change over time, often with more-specific elements being added, so errors of specificity can accrue over time.
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