To determine whether sociotechnical probabilistic risk assessment can create accurate approximations of detailed risk models that describe error pathways, estimate the incidence of preventable adverse drug events (PADEs) with high-alert medications, rank the effectiveness of interventions, and provide a more informative picture of risk in the community pharmacy setting than is available currently.
The complexity, variation in practice patterns, and level of automation of high-technology radiotherapy renders this "one-size-fits-all" prescriptive QA paradigm ineffective or cost prohibitive if the high-probability error pathways of all possible clinical applications of the device are to be covered.
What is more important is the development of a more open mindset and just culture for reporting near misses and incidents, with an increased emphasis on incident learning to uncover latent error pathways.
Given that ANC1 is not epistatic with all members of the error-free branch of postreplicative repair, it was important to determine whether ANC1 acts in an error-free or error-prone pathway with respect to mutagenesis.
DSB repair through HR process is an error free pathway, since it requires an extensive region of sequence homology between the damaged and template strands, whereas NHEJ is an error prone alternate pathway for the repair of DSBs, which essentially joins broken chromosomal ends independent of sequence homology.
Thus, anc1Δ's sensitivity to damage-induced mutagenesis is consistent with Anc1 acting in an error-free rather than an error-prone pathway.
The epistasis of ANC1 with RAD5 does not help us determine to which branch of PRR it belongs, as RAD5 has a role in the error-prone as well as the error-free pathway.
While HR results in an error-free repair, NHEJ is an intrinsically error-prone pathway.
Rad5 appears to play a complex role in these pathways, with evidence for its participation in error-prone translesion synthesis, and at least one putative branch of the error-free pathway, although its primary role is considered to be in the error-free branch [15], [17], [18], [23], [24].
When the error-prone pathway is impaired, cells become refractory to spontaneous and damage-induced mutagenesis; when the error-free pathway is impaired, cells become, if anything, more susceptible to damage-induced mutagenesis.
