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Exact(6)
This error included any error in the sequencing and during PCR.
So the possibility of an error in the sequencing is excluded.
Then our hypothesis is written as (1) (2) In the following models, we assume there is no systematic error in the sequencing.
Having access to clearly unaffected members of the family also allows one to eliminate sources of systematic error in the sequencing data by eliminating from consideration those variants also found in unaffected members [ 39].
Clearly, given the evolutionary rates governing nucleotide change among enteric bacteria combined with the risk of intrinsic polymerase error in the sequencing process itself, each step of the pathway, from isolate collection and template preparation to the sequencing reactions, could potentially spawn artifactual variability.
High error in the sequencing, amplification or synthesis of barcodes, especially for those with limited sequence distance from one another, as well as barcode contamination during handling or mixed clusters/small physical cluster distance on the flow cell can lead to sample misidentification.
Similar(54)
Two methods of studying the statistical error in the sequences of data obtained by computer simulation are compared.
A further source of error in the sequence databases is the prediction of spurious genes (4).
Specifically, a C was added to correct a reading frame error in the sequence agatgatcaacagccattaagaaaggCctcaagaaaaatgaagactgaaaaag.
This suggested that there could be a possible error in the sequence assembly.
To assess error in the sequence data, sequence reads that were at least 99% identical were aligned.
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