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Kind souls suggested there could be an error in the sampling and urged her to be re-tested elsewhere.
This factor (sheep population) has been incorporated, and the corresponding analysis was carried out; see in the "Materials and methods" section "Sheep Population" and the section "Results". In the "Discussion" section the following paragraph was included where we recognized the possibility of guanaco moving as possible source of error in the sampling:.
For these genes, insufficient coverage as the cause for their being missing from the abdominal transcriptome is less parsimonious than the alternative possibility, which is that random error in the sampling from the whole abdomen caused some genes with high local expression to be missing from the composite structure transcriptome.
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Uncertainties in volume fraction may be inclusive to the standard error in the samples graphed in Figures 3a,b and 4a,b.
Better results were achieved by incorporating prior information about error in the sample covariance matrix.
The chance to incorrectly declare an effect because of random error in the sample is called type I error.
F ST estimations assume that there is no error in the sample frequencies, but with many alleles per locus the precision of sample frequencies is questionable.
Although RT enzymes have higher error rates than their PCR counterparts, PCR utilises numerous amplification cycles and as errors are cumulative the amount of PCR error in the sample increases with each cycle.
During the peer review process, we noted an error in the sample size calculation as the SD used reflected an overall VISA-A score rather than a change in score over time.
Accuracy of entry was assured by checking a sample of 5 (10%) of each batch of 50 questionnaires: batches yielding more than 1 error in the sample were returned for re-input of all 50 questionnaires.
A P-value less than 0.05 may be chosen as threshold for statistical significance for the primary outcome, only if 0.05 has been used as the acceptable risk of type I error in the sample size calculation and the sample size has been reached.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com