Sentence examples for error corrected reads from inspiring English sources
Referred to in this paper as Hybrid Assembly, these error corrected reads are more amenable to traditional assembly using overlap graph assemblers like the Celera software package [ 14].
This set of error corrected reads also included a portion of the raw PacBio reads where there was no Illumina coverage and no error correction could be performed.
This study aims to characterize the pineapple chloroplast genome and to compare the pineapple chloroplast to other members of the commelinids clade by using PacBio error corrected reads to perform de novo assembly of the chloroplast genome.
The contig information of each common minke whale sample was generated from error corrected reads using the Allpath-LG algorithm [ 10] and is described in Additional file 1: Table S1.
Most existing NGS assemblers follow the same general outline: break the (possibly error corrected) reads into k-mers, construct the de Bruijn graph on the set of resulting k-mers, simplify the de Bruijn graph, resolve repeats by using mate pair information and construct contigs.
In contrast, the error corrected PacBio reads had the most high GMS bases of any technology, and represents the upperbound on what could be achieved with the pre-error corrected reads.
Following this error correction, corrected reads were sorted on length and downsampled to two different coverage targets.
We have evaluated the performance of ShoRAH (Zagordi et al., 2011) and QuasiRecomb (Zagordi et al., 2012) for simulated consensus error-corrected read data.
In the iterative version, if there has been a substantial amount of changes in the reads, we run the next iteration of error correction; otherwise, output the corrected reads.
A third method of assembly involves using the short, higher accuracy reads generated by Illumina or Life Technologies sequencers to error-correct the long PacBio reads and use those corrected reads for assembly (see Figure 1B).
We therefore downsampled the error-corrected reads targeting two coverages of 12-15× 12-15×-25×.
