All measured P curves exhibit power law behavior ω − α with different exponent values, α = 1.69 ± 0.29 (α min = 1.16 and α max = 2.07 ) where the error captures the cell-to-cell variability (N = 7).
Both the observed results and simulations show similar increases in correlation when thresholding on the number of reads, indicating that modeling counting error captures most of the noise in the cDNA sequencing data ([see Additional file 1, Table S4] and Fig. 1E).
Because our analyses used replicate PCR reactions of starting genomic DNA, high fidelity polymerases, plus sequencing and restriction mapping of total PCR products, we can exclude that the polymorphisms reflect rare in vitro polymerase errors captured by molecular cloning.
Most of them had low coverage and are likely to represent splicing errors, captured due to the high overall coverage in our data set.
Some of the low-abundance events may be splicing errors captured in EST libraries constructed from plant tissues under various growth conditions, so the higher level of low-abundance AS events in Os could indicate higher error rates for the Os spliceosome.
The terminology of "original error" captures the essence that the error is injected to one link from the outside word.
By standardizing code and automating error capture, NanoStringNorm will enable more reproducible and robust analysis of NanoString datasets.
As well as a general error term capturing unobserved variation across GPs, we also included a GP-specific error term to account for the fact that there were multiple observations for each GP.
These factors would effectively have led to measurement error in capturing the true time of onset of symptoms, and the result is likely to have been a bias in our results towards the null.
Quantitative results are shown for the errors in capturing the anisotropy part of the Reynolds stress tensor for several RANS models, including Spalart Almaras, κ−ω SST and transition k−ω SST.
We do not see that larger error and bias estimates raise the possibility of sources of error not captured by the estimates used, though we have carefully examined the literature on the use of the Jarzynski theorem.
