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All qRT-PCR assays are characterised by biological variation, mainly the inherent variability of mRNA levels in different tissues and individuals, but also analytical variations characterised by differences in RNA quality, variability in extraction protocols, RT and PCR efficiencies that can lead to erroneous and confusing results (Huggett et al, 2005).
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And confusing.
"Frustrating and confusing.
Eyewitness reports from the scene were confused and confusing.
Everything is new and confusing.
It's hollow and confusing.
Its ugly and confusing.
It looks stupid and confusing.
It gives an erroneous impression that novel snoRNAs have actually been found and confuses the overall picture.
And confused.
Shattered and confused.
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