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Spontaneous antibiotic-resistant mutants of L. sphaericus or B. amyloliquefaciens were isolated by increasing the concentration of ampicillin (Ap), streptomycin (Sm), chloramphenicol (Cm), kanamycin (Km), rifampicin (Rif), tetracycline (Tc), and erythromycin (Erm) resistance in selective media (Ibrahim et al. 1998).
Strain LAC was made Erm sensitive by serial passage in TSB in order to cure the strain of the native plasmid pUSA03 that confers Erm resistance [45].
An erythromycin (erm) resistance determinant was amplified from pIL253 [ 68], using the primers: 5'-GATATCGAAGCAAACTTAAGAGTGT-3' and 5'-GATATCTACAAAAGCGACTCATAGA-3' using standard conditions and cloned into pCR2.1 (Invitrogen).
The detection of tet and erm resistance genes by qPCR has frequently been used to monitor AR gene loads in different environments [ 13, 39– 41].
qPCR analyses were performed to quantify tetracycline (tet) and erythromycin (erm) resistance genes in the total microbial DNA from the cheese samples.
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MIC – minimal inhibitory concentration; erm – erythromycin resistance methylase; mef – macrolide efflux; rRNA – ribosomal RNA JJ carried out the MIC and molecular genetic studies.
Spo0A: Stage 0 sporulation protein A; erm: Erythromycin resistance; RNAseq: RNA sequencing; SDS-PAGE: Dodecyl sulfate polyacrylamide gel electrophoresis; FDR: False discovery rate; TEM: Transmission electron microscopy.
Persistence of ARBs (such as Escherichia coli) and ARGs [ tet and erm (erythromycin resistance methylase)] has been observed after composting (Sharma et al. 2009), and ARGs can persist even in the absence of selection pressure (Johnsen et al. 2011).
(GABHS): Group A Beta hemolytic Streptococcus; (EES): Erythromycin; (PFGE): Pulse Field Gel Electrophoresis; (MIC): Minimum Inhibitory Concentration; (D- zone test): Double disk diffusion; (CLSI): Clinical Laboratory Standards Institute; (CI): Confidence Interval; (mef): macrolide efflux; (erm): erythromycin resistance methylase.
The most notable phenotypic difference between 630 and 630Δ erm, erythromycin resistance, was found to be the result of a 2.4 kb deletion in the mobile genetic element Tn 5398 that eliminates an ermB gene [ 18].
The presence of macrolide resistance genes erm(B), erm(C), erm(A), erm TR), mef(A) and mrs(A) were determined by PCR as described earlier [ 12].
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