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Gene reporter assays clearly showed that in MCF7 cells both EREs needed to be inactivated to impair p53-dependent transactivation (Figure 1A).
This suggests that E2 needs the wild-type promoter for full transcriptional repression of this gene and it also suggests the presence of putative EREs in the region between −1819/−877 bp of this promoter.
It is recognized that the affinity of receptor binding to an ERE, as measured in vitro, need not correlate with the potency of the enhancer function that is observed [53] and that multiple EREs can work cooperatively in order to enhance target gene transcription [64], [65].
You're hard-working (yeah, hard at working stealing my 13.9% tax money!), patriotic (oh God...h ere comes that "I served in the military" crap again... oy!) and needy ("I'm 90 and need my Social Security and Medicare... wah-wah-wah... ..) and need a little government assistance now and then (and by "now and then" we mean "all the time").
Maybe we need a doula for dying, I reflect as melodious words surface, telling me what I have to do with the life left to be lived: "To love that well, which thou must leave ere long".
Remember what Poor Richard says, Buy what thou hast no Need of, and ere long thou shalt sell thy Necessaries".
"Las git ootay ere".
Ebi Ere added 10 points.
"Ere it's tae late, mon.
Improve your French, and ere you feed.
Th ere is no american temperament.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com