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Taking into account the observed frequencies of truncation and missense mutations (Table 2), and the total length of gene regions of A. thaliana genome (see Methods), we have roughly estimated that each TILL er line contains, on average, 30 genes with knock-out mutations and 281 genes with aminoacid substitutions.
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If EasyEverything has even a smidgen of the success it has had here, queues -- er, lines will begin to form around the super-cybercafe before it even opens.
We evaluated both Col and L er lines introgressed with various FRI and FLC alleles and hybrids between these lines and C24.
This relationship is supported by the larger ratios observed in L er lines with high mutation density generated with EMS doses ≥35 mM, than in lines with lower density obtained with 25-30 mM EMS.
In a recent study, it was reported that tamoxifen inducible Cre-ER line has toxicity in hematopoiesis and the increase of apoptosis in many tissues during embryonic development, and the toxicity also depends on the injection dose of tamoxifen [37].
Initially, twelve clonal cell lines, MDA ER α line 1 to MDA ER α line 12, were selected, tested, and found to express ER α protein (data not shown).
We also chose ER α line 7 cells (MDA-ER α7) as a slower growing cell line for additional testing.
The ER α line 5 cells (MDA-ER α5) was selected as a faster growing cell line for further testing.
The ER marker line ER-gk was obtained from the Arabidopsis Biological Resource Center (ABRC) [ 74].
In addition, activation of Akt in the conditionally inducible Myr+Akt:ER cell line blocked transcriptional upregulation of Mirk.
In vitro studies suggest that ER+ breast cancer cell lines are generally more sensitive than ER– cell lines to 1,25(OH)2D–mediated growth regulation (Welsh et al. 2002).
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