Alignment accuracy was high for both STAR and TopHat: in ER TopHat aligned 94.4% (respectively 92.63%) of the bases accurately when aligned with (respectively without) gene models.
This is especially critical for L er and Cvi, where genome information is more limited than for the reference accession Col. Figure 2A shows an example where three haplotypes appear to be present at a single locus when the short read data of L er are aligned against the Col reference genome.
No, the actual most fascinating Twitter account is @rejectpetitions, a feed which – as far as I know – has no interest in "bench-pressing controversy" by, er, openly aligning itself with the powers-that-be (ah, what breathtaking vistas of possibility "free thought" can open up!), and instead just gets on with the serious business of listing all the e-petitions the UK government has rejected.
We found that HeLa-prME, but not control HeLa cells (data not shown), displayed dilated ER membranes with aligned round particles and elongated parallel tubules.
However, around 3.9% of these regions featured less than 5% confidently aligned positions, when aligning short reads of L er against the reference sequence.
Three distinct L er haplotypes align to this region, indicating that this sequence is present in triplicate in the L er genome.
As L er is homozygous, at least two haplotypes were not aligned to their respective origin.
(A ) The percentage of confidently aligned positions within the resequencing of L er was used as proxy for local sequence similarity.
DRs or ERs in an accurate depth map should be exactly aligned with the objects' boundaries in the color video.
With the aim to identify a conserved ER targeting motif in the cytosolic tails of the different GCSI cloned so far, we aligned their sequences (Table 1).
